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課程名稱 |
重組蛋白質生產技術
PRODUCTION OF RECOMBINANT PROTEINS |
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開課學期 |
95-2 |
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授課對象 |
生物資源暨農學院 生物科技研究所 |
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授課教師 |
劉?睿 |
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課號 |
Biot7001 |
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課程識別碼 |
642EM0020 |
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班次 |
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學分 |
2 |
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全/半年 |
半年 |
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必/選修 |
選修 |
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上課時間 |
星期三5,6(12:20~14:10) |
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上課地點 |
生技所R209 |
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備註 |
本課程以英語授課。
限碩士班以上
總人數上限：20人
外系人數限制：19人 |
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課程簡介影片 |
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核心能力關聯 |
核心能力與課程規劃關聯圖 |
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課程大綱
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課程概述 |
週數 日期 課程大綱
1. 02/28 228 Memorial Day
2. 03/07 Introduction
3. 03/14 Principles of recombinant protein production
4. 03/21 Production of recombinant proteins by Escherichia coli
5. 03/28 Production of recombinant proteins by Pseudomonas fluorescens
6. 04/04 Production of recombinant proteins by Staphylococcus carnosus and other Gram-positive Bacteria
7. 04/11 Production of recombinant proteins by Arxula adeninivorans
8. 04/18 Midterm examination
9. 04/25 Production of recombinant proteins by Hansenula polymorpha
10. 05/02 Production of recombinant proteins by Pichia pastoris
11. 05/09 Production of recombinant proteins by Yarrowia lipolytica
12. 05/16 Production of recombinant proteins by Aspergillus sojae
13. 05/23 Production of recombinant proteins by Sordaria macrospore
14. 05/30 Production of recombinant proteins by Mammalian Cells
15. 06/06 Production of recombinant proteins by Plant Cells
16. 06/13 Presentations and colloquial discussions
17. 06/20 Terminal examination |
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課程目標 |
The course is aimed at providing a comprehensive view of the newly identified and defined recombinant protein production systems, including the two Gram-negative organisms (E. coli and Pseudomonas fluorescens), the Gram-positive Staphylococcus carnosus, four yeast species (Arxula adeninivorans, Hansenula polymorpha, Pichia pastoris and Yarrowia lipolytica), and the two filamentous fungi Aspergillus sojae and Sordaria macrospora. The description of these microbial platforms is further supplemented by an overview on expression in mammalian and plant cells. |
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課程要求 |
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預期每週課後學習時數 |
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Office Hours |
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指定閱讀 |
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參考書目 |
1. Gellissen, G. 2005. Production of recombinant proteins. WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany.
2. Vaillancourt, P. E. 2003. E. coli Gene expression protocols. Humana Press, Totwa, NJ, USA.
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評量方式
(僅供參考) |
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